Postdoc in Analysis of N-WASP dependent regulation of senescence in keratinocytes

Postdoc in Analysis of N-WASP dependent regulation of senescence in keratinocytes 
at BRIC 
Faculty of Health and Medical Sciences 
University of Copenhagen, Denmark 
 
We are looking for a highly motivated and dynamic researcher for a 1 year post doc position starting in May 2019 in the Brakebusch lab. The Brakebusch lab is located at the Biotech Research and Innovation Center (BRIC) (www.bric.ku.dk/Research/Brakebusch_Group/), which is a Center of Excellence in biomedical research with around 200 scientists. 
 
Our research 
N-WASP is a ubiquitously expressed actin-nucleating molecule present in the cytoplasm and in the nucleus. Recently, we revealed that nuclear N-WASP is an important epigenetic regulator controlling the expression of IL-23 in keratinocytes. Mice lacking N-WASP in keratinocytes show perturbed e regulation of the epigenetic mark H3K9me2 and develop an IL-23 dependent skin inflammation (Li et al., 2018, Nat. Commun.). Unpublished data from our group indicate now that N-WASP is also important for the development of skin tumor by regulating keratinocyte senescence in vivo and in vitro. Aim of the current project is to investigate the structure-function relationship of N-WASP with respect to senescence regulation by expressing N-WASP point mutations in primary mouse keratinocytes. 
 
Your job 
Your task would be to generate 10 point mutants of N-WASP and express them in primary mouse keratinocytes lacking N-WASP. Senescence development will be monitored by proliferation assays and detection of different senescence markers by qRT PCR and Western blot. Furthermore, epigenetic changes and N-WASP protein-protein interactions will be tested.  
 
Profile  
We are looking for a highly motivated and enthusiastic scientist with the following competencies and experience: 
 
Essential experience and skills: 
  • You have a PhD in biological/biomedical science 
  • You are highly experienced in cell culture and molecular biology 
  • You have an active interest in cellular senescence, cancer, and aging 
  • Proficient communication skills and ability to work in teams 
  • Excellent English skills written and spoken 

Desirable experience and skills: 

  • Experience in isolation and culture of primary keratinocytes  
  • Experience in CRISPR genome editing 
 
Place of employment 
The place of employment is at BRIC, University of Copenhagen. We offer creative and stimulating working conditions in dynamic and international research environment. Our research facilities include modern laboratories and core facilities and an exciting research environment with ambitious researchers from all over the world. 
 
Terms of employment  
The employment as postdoc is a full time and fixed-term position for 1 year. Starting date is May 1, 2019, or after agreement. 
Salary, pension and terms of employment will be in accordance with the agreement between the Danish Ministry of Finance and AC (Danish Confederation of Professional Associations). Currently, the monthly salary starts at 33,700 DKK/ approx. 4,514 Euro (October 2018-level). Depending on qualifications, a supplement may be negotiated. The employer will pay an additional 17.1 % to your pension fund. 
 
Non-Danish and Danish applicants may be eligible for tax reductions, if they hold a PhD degree and have not lived in Denmark the last 10 years. 
 
The position is covered by the “Memorandum on Job Structure for Academic Staff at the Universities” of June 28, 2013. 
 
Questions 
For further information please contact Cord Brakebusch (cord.brakebusch@bric.ku.dk)   
Foreign applicants may find this link useful: www.ism.ku.dk (International Staff Mobility).  
 
Application procedure 
Your online application must be submitted in English by clicking ‘Apply now’ below. Furthermore, your application must include the following documents/attachments – all in PDF format: 
 
1. Motivated letter of application (max. one page). 
2. CV incl. education, work/research experience, language skills and other skills relevant for the position. 
3. A certified/signed copy of a) PhD certificate and b) Master of Science certificate. If the PhD is not completed, a written statement from the supervisor will do. 
4. List of publications. 
 
Application deadline: 28 February, 2019, 23.59pm CET  
 
We reserve the right not to consider material received after the deadline, and not to consider applications that do not live up to the abovementioned requirements. 
 
The further process  
After the expiry of the deadline for applications, the authorized recruitment manager selects applicants for assessment on the advice of the hiring committee. All applicants are then immediately notified whether their application has been passed for assessment by an unbiased assessor. Once the assessment work has been completed each applicant has the opportunity to comment on the part of the assessment that relates to the applicant him/herself.  
 
You can read about the recruitment process at http://employment.ku.dk/faculty/recruitment-process/
 
The applicant will be assessed according to the Ministerial Order no. 242 of 13 March 2012 on the Appointment of Academic Staff at Universities.  
 
Interviews are expected to be held in week 13. 
 
University of Copenhagen wish to reflect the diversity of society and welcome applications from all qualified candidates regardless of age, disability, gender, nationality, race, religion or sexual orientation. Appointment will be based on merit alone. 
 
SØG STILLINGEN

Københavns Universitet giver sine knap 10.000 medarbejdere muligheder for at udnytte deres talent fuldt ud i et ambitiøst, uformelt miljø. Vi sikrer traditionsrige og moderne rammer om uddannelser og fri forskning på højt internationalt niveau. Vi søger svar og løsninger på fælles problemer og gør ny viden tilgængelig og nyttig for andre.

Kontakt

Cord Herbert Brakebusch

Info

Ansøgningsfrist: 28-02-2019
Ansættelsesdato: 01-05-2019
Arbejdstid: Fuldtid
Afdeling/Sted: BRIC

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